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Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.
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Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.
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Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.
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Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.
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Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.
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Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.
Human Growth Factor Antibody Array 1, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.
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Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.
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Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.
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Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.
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Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.
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Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.

Journal: BioMed Research International

Article Title: Anti-Inflammatory and Protective Effects of Juncus effusus L. Water Extract on Oral Keratinocytes

doi: 10.1155/2022/9770899

Figure Lengend Snippet: Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.

Article Snippet: Chemokine detection in cell culture supernatants obtained from RT-7 cells treated with P. gingivalis LPS and/or the J. effusus L. water extract was performed using the Human Chemokine Antibody Array 1 (Ray Biotech, Inc., Norcross, GA, USA), according to the manufacturer's instructions.

Techniques: Cell Culture, Ab Array